Breeding two complete

Second breeding went well today.  Rylee knew what was up and got onto the table and stood pretty still for the 10 min procedure.  We should know in about 24 days if there are puppies. 

A little article on insemination written by Dr. Hutchinson, whose facilty I went to for Rylee's breedings.

If both breeding participants are present, fertile and ovulation timed ready, a natural breeding is performed. If for some reason a natural cannot be achieved, then a vaginal artificial insemination is used to breed the bitch. The technique requires specific procedures be performed.
The male's semen is collected. This is achieved by manual stimulation. The collector needs to obtain the second fraction of the ejaculate (the milky, sperm rich portion) stopping the collection when the third fraction starts (watery in appearance) which consists of prostatic fluid.

The bitch should have her rear end elevated for the artificial insemination procedure. This positioning facilitates the semen's deposition and flow to the cervical opening, a necessary for the semen being in position to be "pumped" into the uterus. The inseminator should digitally stoke the roof of the vaginal tract (also called "feathering") for 1-2 minutes after insemination. This technique simulates the tie of a natural breeding. The bitch's rear should be elevated for 2-3 minutes post-insemination.
The necessity to bypass the cervix and place the semen into the uterine lumen is beneficial in improving the conception rates in numerous situations. These include the use of frozen semen, fresh cooled semen, poor semen quality and in situations where examination of the bitch's uterus is desired.
Two methods are used to achieve the intrauterine deposition of semen, the transcervical insemination and the surgical insemination. These techniques each have their own usage guidelines and one does not replace the other as has been mistakenly represented to dog breeders.

The transcervical insemination (TCI) is performed with the bitch in a standing position. No sedation nor anesthesia is required. A fiber optic cystourethoscope is used vaginally to visualize the opening to the cervix. A flexible catheter is maneuvered through the cervix into the uterus. It is important that the breeder realize that the veterinarian is not visualizing the inside of the uterus and this technique does not allow for evaluation of the uterus.
The TCI procedure is visualized on a television monitor and does allow for examination of the vaginal tract, however. The semen is gently pushed through the catheter from a syringe. The veterinarian can visualize that the semen flows easily into the uterus and does not flow back into the vaginal tract.